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《环境科学与工程前沿(英文)》 2022年 第16卷 第10期 doi: 10.1007/s11783-022-1541-8
● Perfluorooctanesulfonic acid and perfluorooctanoic acid highest in human milk.
关键词: Human biomonitoring Human breast milk LC-MS/MS analysis Lifestyle parameters
《医学前沿(英文)》 2021年 第15卷 第6期 页码 942-942 doi: 10.1007/s11684-021-0876-z
Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice
Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI
《农业科学与工程前沿(英文)》 2014年 第1卷 第3期 页码 179-184 doi: 10.15302/J-FASE-2014020
关键词: recombinant human butyrylcholinesterase (rhBCHE) human lactoferrin bacterial artificial chromosome (hLF BAC) transgenic mice milk
null
《医学前沿(英文)》 2016年 第10卷 第1期 页码 41-51 doi: 10.1007/s11684-016-0429-z
Midline2 (MID2) is an ubiquitin-conjugating E2 enzyme linked to tumor progression and a novel interacting partner of breast cancer 1, early-onset (BRCA1). However, the role of MID2 in breast cancer remains unknown. This study investigated the expression, prognostic value, and role of MID2 in breast cancer. The expression of MID2 mRNA and protein was significantly upregulated in breast cancer tissue and established cell lines compared with that in normal breast epithelial cells and paired adjacent non-tumor tissue (P<0.001). Immunohistochemical analysis demonstrated that MID2 was overexpressed in 272 of 284 (95.8%) paraffin-embedded, archived breast cancer tissue. Moreover, MID2 expression increased with advanced clinical stage (P<0.001). High MID2 expression was significantly associated with advanced clinical stages and T, N, and M staging (all P<0.05). Univariate and multivariate analyses indicated that high MID2 expression was an independent prognostic factor for poor overall survival in the entire cohort (93.73 vs. 172.1 months; P<0.001, log-rank test) and in subgroups with stages Tis+ I+ II and III+ IV. Furthermore, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide colony formation, and anchorage-independent growth ability assays were conducted. Results showed that siRNA silencing of MID2 expression significantly reduced MCF-7 and MDA-MB-231 cell proliferation in vitro and blocked the growth of MDA-MB-231 cell xenograft tumors in vivo (P<0.05). This study indicated that MID2 may be a novel prognostic marker and interventional target in breast cancer.
关键词: breast cancer MID2 proliferation overall survival xenograft
Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI
《农业科学与工程前沿(英文)》 2018年 第5卷 第3期 页码 382-389 doi: 10.15302/J-FASE-2018211
The mammary gland provides a novel method for producing recombinant proteins in milk of transgenic animals. A key component in the technology is the construction of an efficient milk expression vector. Here, we established a simple method to construct a milk expression vector, by a combination of homologous recombination and digestion-ligation. Our methodology is expected to have the advantages of both plasmid and bacterial artificial chromosome (BAC) vectors. The BAC of mouse whey acidic protein gene (mWAP) was modified twice by homologous recombination to produce a universal expression vector, and the human lysozyme gene (hLZ) was then inserted into the vector by a digestion-ligation method. The final vector containing the 8.5 kb mWAP 5′ promoter, 4.8 kb hLZ genomic DNA, and 8.0 kb mWAP 3′ genomic DNA was microinjected into pronuclei of fertilized mouse embryos, to successfully generate two transgenic mouse lines that expressed recombinant human lysozyme (rhLZ) in milk. The highest expression level of rhLZ was 0.45 g·L−1, and rhLZ exhibited the same antibacterial activity as native hLZ. Our results have provided a simple approach to construct a universal milk expression vector, and demonstrated that the resulting vector regulates the expression of hLZ in milk.
关键词: BAC recombinant methods gene expression human lysozyme transgenic mice milk expression vector
Wenjie Zhu, Binghe Xu
《医学前沿(英文)》 2021年 第15卷 第2期 页码 208-220 doi: 10.1007/s11684-020-0795-4
关键词: endocrine-resistant HR+/HER2- advanced breast cancer randomized clinical trials meta-analysis targeted therapy
Global warming potential associated with Irish milk powder production
William Finnegan, Jamie Goggins, Aksana Chyzheuskaya, Xinmin Zhan
《环境科学与工程前沿(英文)》 2017年 第11卷 第3期 doi: 10.1007/s11783-017-0949-z
关键词: Dairy Global warming potential Ireland Life cycle assessment Milk powder Milk production
Progress in systemic therapy for triple-negative breast cancer
Hongnan Mo, Binghe Xu
《医学前沿(英文)》 2021年 第15卷 第1期 页码 1-10 doi: 10.1007/s11684-020-0741-5
关键词: triple-negative breast cancer immunotherapy targeted therapy
How to judge the association of postmenopausal hormone therapy and the risk of breast cancer
Ling XU
《医学前沿(英文)》 2010年 第4卷 第3期 页码 290-293 doi: 10.1007/s11684-010-0093-7
关键词: breast cancer postmenopausal hormone therapy unopposed estrogen therapy combined estrogen-progestin therapy
Metabolism and immunity in breast cancer
Deyu Zhang, Xiaojie Xu, Qinong Ye
《医学前沿(英文)》 2021年 第15卷 第2期 页码 178-207 doi: 10.1007/s11684-020-0793-6
Qiong DAI MD, Bei LIU MD, Yukai DU MM,
《医学前沿(英文)》 2009年 第3卷 第4期 页码 452-458 doi: 10.1007/s11684-009-0080-z
关键词: meta-analysis breast cancer risk factors reproductive factors oral contraceptive use
Expression of PC-cell-derived growth factor in breast cancer
Haiping SONG MD, Lan SHI MD, Chunping LIU MD, Tao HUANG MD,
《医学前沿(英文)》 2009年 第3卷 第4期 页码 426-430 doi: 10.1007/s11684-009-0085-7
关键词: PC-cell-derived growth factor breast neoplasms clinical markers
A case of primary neuroendocrine breast carcinoma that responded to neo-adjuvant chemotherapy
null
《医学前沿(英文)》 2015年 第9卷 第1期 页码 112-116 doi: 10.1007/s11684-014-0345-z
Primary neuroendocrine breast carcinoma (NEBC) is a very rare type of breast cancer. Two characteristic biomarkers, namely, CgA and Syn, should be immunohistochemically detected to diagnose NEBC. In this study, a 43-year-old woman with a large mass of 8.3 cm × 2.9 cm in her right breast was reported. The patient was pathologically diagnosed with NEBC after specific markers, including CgA and Syn, as well as few differential markers, such as CK7, ER, PR, C-erbB-2, NSE, and E-cadherin, were immunohistochemically detected. The patient showed a remarkable response to four cycles of neo-adjuvant chemotherapy (partial response based on RECIST criteria) and sequentially underwent modified radical mastectomy. Moreover, the diagnosis and treatment of NEBC based on this case and available related literature were discussed.d literature were discussed.
《医学前沿(英文)》 页码 907-923 doi: 10.1007/s11684-023-1009-7
Jacqueline CONCHA, Caroline WEINSTEIN, María Elvira Zú?IGA
《化学科学与工程前沿(英文)》 2013年 第7卷 第4期 页码 482-489 doi: 10.1007/s11705-013-1342-5
关键词: pectic extracts antiproliferative activity breast cancer enzymatic treatment
标题 作者 时间 类型 操作
Perfluoroalkane acids in human milk under the global monitoring plan of the Stockholm Convention on Persistent
期刊论文
Midline2 is overexpressed and a prognostic indicator in human breast cancer and promotes breast cancer
期刊论文
Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice
Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI
期刊论文
Midline2 is overexpressed and a prognostic indicator in human breast cancer and promotes breast cancer
null
期刊论文
Construction of a universal recombinant expression vector that regulates the expression of human lysozymein milk
Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI
期刊论文
Overcoming resistance to endocrine therapy in hormone receptor-positive human epidermal growth factor
Wenjie Zhu, Binghe Xu
期刊论文
Global warming potential associated with Irish milk powder production
William Finnegan, Jamie Goggins, Aksana Chyzheuskaya, Xinmin Zhan
期刊论文
How to judge the association of postmenopausal hormone therapy and the risk of breast cancer
Ling XU
期刊论文
Meta-analysis of the risk factors of breast cancer concerning reproductive factors and oral contraceptive
Qiong DAI MD, Bei LIU MD, Yukai DU MM,
期刊论文
Expression of PC-cell-derived growth factor in breast cancer
Haiping SONG MD, Lan SHI MD, Chunping LIU MD, Tao HUANG MD,
期刊论文
A case of primary neuroendocrine breast carcinoma that responded to neo-adjuvant chemotherapy
null
期刊论文
frequency of alternative splicing variants of the oncogene in neuroendocrine tumors of the pancreas and breast
期刊论文